The purpose of the 16th International Artificial Intelligence and Cyber Security Workshop (AICS2023) is to raise awareness of cybersecurity, promote the potential of industrial applications, and give young researchers exposure to the main issues related to the topic and ongoing works in this area. AICS2023 will provide a forum for researchers, security experts, engineers, and research students to demonstrate new technologies, present the latest research works, share ideas, and discuss future directions in the fields of artificial intelligence and cybersecurity.